Possession of exotic animals and zoonotic diseases: an approach from the Mexican context

The possession of exotic animals as pets is a social practice that has become more visible in Mexico in recent years, so it is interesting to understand its environmental and social implications and those related to human health. The present study aims to identify the main species of exotic animals kept as pets and the zoonotic diseases reported in these species. We analyzed official figures of seizures of exotic fauna in Mexico and reviewed specialized literature on zoonotic diseases documented in Mexico in these species. We identified zoonoses in species of fauna that can be acquired legally and illegally in the country, reported in environments in which animals coexist with other species and are in direct contact with people, which represents an important factor in the spread and propensity of this type of disease. We conclude that the sanitary regulation of wildlife markets, the monitoring and studying microorganisms associated with wildlife are valuable strategies to prevent the emergence of zoonoses.

The escalating global problem of accidental human-mediated transport of alien species: A case study using alien herpetofauna interceptions in New Zealand

The global problem of biological invasions will continue escalating, given inadequate biosecurity worldwide. Developing stringent biosecurity is hindered by the lacking essential information on the global flows of alien species, especially alien species accidentally transported and neglected by biosecurity due to inapparent economic significance. We provide evidence and new perspectives on the temporal, geographical, taxonomic, and transport sub-pathway dimensions of the global flows of neglected alien species, using alien amphibians and reptiles ("herpetofauna”) accidentally transported to New Zealand as a case study (2610 records from 1929 to 2021). We decomposed and forecasted the alien herpetofauna transport frequency using locally-weighted smoothing and dynamic regression modelling. We explored geographical patterns of the alien herpetofauna origins and destinations, and explored temporal trends in species diversity. Finally, we analysed a species×transport sub-pathway network to elucidate the diversity of sub-pathways used by alien herpetofauna. Alien herpetofauna transport frequency is generally increasing, with fluctuations coinciding with changes in biosecurity and economic expansion and recessions. The most recent decline was during the COVID-19 recession, but we forecast transport to recover. Two hundred and forty-three alien herpetofauna worldwide arrived at ports of entry across New Zealand. Alien herpetofauna were accidentally transported through 13 sub-pathways, primarily as stowaways in 'personal effects and household goods', and in 'machinery, vehicles, and equipment'. Our study illuminates that neglected alien species' transport frequency, spatial extent of origins and destinations, species diversity, and accidental transport sub-pathways are hugely underestimated and dynamic. These crucial oversights in the global flows of alien species significantly impede biosecurity worldwide.

Analysis of intermediate hosts and susceptible animals of SARS-CoV-2 by computational methods

COVID-19, a disease caused by SARS-CoV-2 that produces major symptoms of pneumonia, has been a disaster worldwide. The traceability of SARSCoV- 2 and the discovery of susceptible animal species is crucial to halt viral transmission and explore the mechanism of cross-species transmission. We selected 82 representative ACE2 sequences from the 1000 sequences with the closest homology to the hACE2 protein. All selected ACE2 proteins were subjected to homology modeling. Potential natural and intermediate hosts, as well as animal species susceptible to SARS-CoV-2, were analyzed systematically by calculation of the binding free energy of ACE2 protein to the RBD of SARSCoV- 2. Primates, some wild Felidae, civets, goats, spotted hyenas and golden hamsters are susceptible to SARS-CoV-2 and may be potential intermediate hosts, whereas pangolins, birds and reptiles are unlikely to be intermediate hosts. Mice, rats and guinea pig are not susceptible to SARS-CoV-2. Given their possible susceptibility, non-human primates, goats and golden hamsters could potentially be used as experimental models to examine SARS-CoV-2 infection without transgenesis. Herein, possible candidates for the natural and intermediate hosts of SARS-CoV-2 are suggested, to provide guidance for subsequent studies.

One Shell of a Problem: Cumulative Threat Analysis of Male Sea Turtles Indicates High Anthropogenic Threat for Migratory Individuals and Gulf of Mexico Residents

Human use of oceans has dramatically increased in the 21st century. Sea turtles are vulnerable to anthropogenic stressors in the marine environment because of lengthy migrations between foraging and breeding sites, often along coastal migration corridors. Little is known about how movement and threat interact specifically for male sea turtles. To better understand male sea turtle movement and the threats they encounter, we satellite-tagged 40 adult male sea turtles of four different species. We calculated movement patterns using state-space modeling (SSM), and quantified threats in seven unique categories;shipping, fishing, light pollution, oil rigs, proximity to coast, marine protected area (MPA) status, and location within or outside of the U.S. Exclusive Economic Zone (EEZ). We found significantly higher threat severity in northern and southern latitudes for green turtles (Chelonia mydas) and Kemp’s ridleys (Lepidochelys kempii) in our study area. Those threats were pervasive, with only 35.9% of SSM points encountering no high threat exposure, of which 47% belong to just two individuals. Kemp’s ridleys were most exposed to high threats among tested species. Lastly, turtles within MPA boundaries face significantly lower threat exposure, indicating MPAs could be a useful conservation tool.

Thailand's online reptile market decreases but shifts toward native species during COVID-19.

The recent COVID-19 pandemic presented the world with a crisis of incredible scale and made wildlife markets the focal point of authorities. Scientific literature on COVID-19 and wildlife trade overwhelmingly focused on the zoonotic risks of wildlife markets. As many physical marketplaces for wildlife were faced with closure or restrictions to curb the spread of COVID-19, alternative sale platforms were sought. I monitored social media platforms in Thailand during the pandemic and compared this with data obtained in 2016. I found a significant reduction of lizards and snakes offered for sale on social media, compared with before the pandemic. Although the quantity decreased, I found that the number of species almost doubled in snakes, of which unprotected native species increased by 245%. Transport restrictions would limit the mobility of harvesters and interrupts trade chains, and thus could explain the reduced number of snakes and lizards for sale. However, the increase in native species for sale shows that the impact of this international trade disruption could shift focus from international trade to what is locally available. Potentially having serious consequences for the conservation of local species and in line with previous studies documenting increased poaching rates and wildlife crime incidents.

Conservation and cultural intersections within Hong Kong's snake soup industry

Snake soup continues to be an iconic tradition in Cantonese culture. Yet little is known about the relationship between snake soup consumption in Hong Kong, wild snake populations, and the communities depending on this tradition for their livelihoods. We applied an interdisciplinary approach including interviews with shopkeepers and genetic analyses of snake meat samples to determine the species consumed in Hong Kong, their source locations, and shopkeepers' views on the future of the industry. We genetically identified the common rat snake Ptyas mucosa, widely distributed throughout East and Southeast Asia, and the Javan spitting cobra Naja sputatrix, endemic to Indonesia, as the species most commonly consumed, which was consistent with interview responses. According to interviews, snakes had mostly been imported from mainland China in the past, but now tend to be sourced from Southeast Asia, particularly Indonesia. Interviews also revealed a pessimistic outlook on the continuation of this tradition because of various factors, including a lasting yet misinformed association of snakes with the 2002-2003 outbreak of severe acute respiratory syndrome. Given the COVID-19 pandemic and China's ensuing ban on the consumption of terrestrial wildlife, Hong Kong's snake soup industry will probably continue to rely on Southeast Asian sources to persist. Given the cultural and conservation issues surrounding this tradition, further research on the economic, ecological and social consequences of snake consumption is needed to examine the broader implications of snake soup and similar industries in the region.

Management and Mapping Ecosystem Services in a Privately Owned Natura 2000 Site: An Insight into the Stellantis–La Mandria Site (Italy)

The Natura 2000 network is an ecological network covering the whole territory of the European Union to ensure the long-term maintenance of threatened or rare natural habitats and species of flora and fauna, including in metropolitan and rural areas. Some of the Natura 2000 sites have been subject to changes in ownership that have led to a fragmentation of the territory. Private entities may own areas within a Natura 2000 site and must ensure sustainable management of their property, especially from an ecological point of view. The case study is the Stellantis–La Mandria site, a private area owned by Stellantis, within the Natura 2000 site “ZSC IT1110079 La Mandria”, near Turin. The research proposes a participatory methodology, mainly addressed in this first phase to experts and professionals and aimed at the valorisation and management of private Natura 2000 sites previously considered as industrial sites, to allow a careful fruition and safeguarding of the natural heritage. The aim of the research is to provide a methodological approach and the first qualitative results useful to providing the owners with an indication for a more targeted management of the site;mapping the areas that provide ecosystem services (ES, especially cultural ones);mapping the areas that could be subject to future fruition;collaborating with the managing body to collect useful data for the future drafting of a new area plan. Two main research activities were carried out, a SWOT (strengths, weaknesses, opportunities, and threats) analysis concerning the management and possible future fruition of the site and a participatory mapping of ES. It should be emphasised that the research allowed the first results to be obtained, which, on the one hand, make it possible to validate the methodology used to achieve the objectives and, on the other hand, the results will have to be implemented over time by involving numerous stakeholders among those who can access the private area. The results of the research highlight opportunities and threats with regard to the conservation of ecological–environmental characteristics and future fruition of the site. The participatory mapping identifies areas with different ecological value and, therefore, different management needs and identifies areas that could be used differently for future fruition. Overall, the results aim to meet some of the European Commission’s wishes regarding Natura 2000 sites, with particular reference to the involvement of different stakeholders and experts for the conservation of biodiversity and the integration of ecological and social aspects into the management and fruition plans.

Healing of Skin Biopsies in Wild Juvenile Green Turtles, Chelonia mydas

Skin biopsies are routinely collected from sea turtles for a variety of molecular analyses;however, almost no information exists on the natural healing rates at the site of the biopsy. Here, we monitored the healing rates of 17 juvenile green turtles (Chelonia mydas) for up to 488 d after we took a 6-mm biopsy tissue sample in Eleuthera, The Bahamas. Although scarring persisted for several months, biopsy sampling had a minimal long-term impact—tissue repair and maturation was observed after a year and a half, and there was no evidence of infection at any point during the healing process.

Illegal Wildlife Trade and Emerging Infectious Diseases: Pervasive Impacts to Species, Ecosystems and Human Health.

Emerging infectious disease (EID) events can be traced to anthropogenic factors, including the movement of wildlife through legal and illegal trade. This paper focuses on the link between illegal wildlife trade (IWT) and infectious disease pathogens. A literature review through Web of Science and relevant conference proceedings from 1990 to 2020 resulted in documenting 82 papers and 240 identified pathogen cases. Over 60% of the findings referred to pathogens with known zoonotic potential and five cases directly referenced zoonotic spillover events. The diversity of pathogens by taxa included 44 different pathogens in birds, 47 in mammals, 16 in reptiles, two in amphibians, two in fish, and one in invertebrates. This is the highest diversity of pathogen types in reported literature related to IWT. However, it is likely not a fully representative sample due to needed augmentation of surveillance and monitoring of IWT and more frequent pathogen testing on recovered shipments. The emergence of infectious disease through human globalization has resulted in several pandemics in the last decade including SARS, MERS, avian influenza H1N1,and Ebola. We detailed the growing body of literature on this topic since 2008 and highlight the need to detect, document, and prevent spillovers from high-risk human activities, such as IWT.